C.F.
A lactation consultant at Illinois Masonic hospital told me freshly expressed breast milk can be stored at room temp for up to 8 hours, can be stored in the refrigerator for up to five days then put in freezer on the fifth day, and can be in the freezer for up to three months. As far as the nutritional value, I've heard there is a slight decrease in nutritional value, but not enough to make it worth throwing it out instead of freezing or using formula. Hope that was helpful.