You are kidding right? She's TWO!
Just so that you don't feel alone.... ALL two year old's don't want to mind their parents. They are at the development stage where they are testing boundaries and challenging authority while still learning and challenging their own abilities through the whole process. Let her do what she can on her own- safely. For example- walking up the stairs by herself (with you behind her of course!), stirring dinner and helping make it, making choices regarding 30 minute dvd programs or playing games.
The MOST IMPORTANT part of raising an independent toddler is to stay consistent. You can not expect a 2 year old to comprehend that "going to their room" is a punishment. Let me guess... she has sleeping issues as well. I would if I thought my room was a bad place to be at the age of 2! Use time out.
Find a small and better yet uncomfortable chair or stool for her to sit in for 2 minutes (one minute per year of age). Take her to the time out chair when she has made a poor behavior choice and sit her in it. She WILL CRY! It is OK! Tell her (at eye level)in a soft but firm voice WHY she is being placed in time out. Set the timer for 2 minutes and stay close- but do not talk to her or engage her in any way. If she leaves the chair, kindly place her back in and say (again in a kind but firm voice) you are in time out. I do not recommend that you leave the room because she may get out and wander off and the whole process will be void. When the timer beeps approach her at eye level (again in a kind but firm voice) explain WHY she was in time out and ask her if she understands. Tell her that you do not accept the behavior and tell her that you love her. Ask for an apology and a hug. Let her go and change the tone of the conversation to a much more positive and happy voice. Smile and go on playing.
KEEP CONSISTANT!
If she does the unwanted behavior again send her back to time out- going through the steps again. YOU CAN DO IT!
We are all in the same boat! Toddlers are toddlers are toddlers! Across the globe. Just remember that she is yours and she will be shaped by how you respond to her behaviors. Let her form her own personality, but along with your husband (remember he is your partner in this "raising a child thing") decide what you deem to be poor behavior choices and be consistent in disciplining those behaviors.